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Image Search Results
Journal: Scientific Reports
Article Title: Trehalose prevents aggregation of exosomes and cryodamage
doi: 10.1038/srep36162
Figure Lengend Snippet: ( A ) Particle size distribution of beta-ELVs measured by nanoparticle tracking analysis. The size distribution curve fits average particle size and concentration. Data from one representative sample out of twelve are shown. ( B ) Western blot analysis of 15 μg of total cell lysates (MIN6) or beta-ELV proteins reveal the presence of canonical exosomal protein markers and the absence of cellular calnexin. ( C , D ) Experion automated electrophoresis analysis of ( C ) protein profiles under reducing conditions, and ( D ) total RNA isolated from MIN6 cells (upper panel) and of beta-ELVs in PBS or TRE (lower panel). ( E ) Cryo-electronic tomography images of fully hydrated, unstained beta-ELV isolated in PBS or TRE show a population of membrane vesicles of heterogeneous shape and sizes. Scale bars: 100 nm.
Article Snippet: For blotting with
Techniques: Concentration Assay, Western Blot, Electrophoresis, Isolation, Tomography, Membrane